Mitochondrial Protein Degradation in Health and Longevity

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We are interested in understanding the molecular and cell biology that underlies aging. To do this, we use fruit fly as a model organism. A current idea for what drives aging is that organisms are unable to maintain function due to a buildup of damaged biomolecules (like proteins). Mitochondria are particularly important organelles for aging--we think mitochondria are the source of free radicals that cause damage that results in aging, and mitochondria are also the organelle that is expected to be most affected by free radical damage.

Our lab is interested in investigating the role that mitochondrial protein damage plays in aging. Recently, we found that some flies that are healthier and live longer have more robust mitochondrial protein degradation. We don't yet know if this is something that is common to all healthier/longer lived flies. During the summer, we are interested in overexpressing genes that are expected to increase protein degradation in mitochondria.  Assessing their effects on mitochondrial functions (i.e. mitochondrial protein degradation, Krebs cycle, electron transport, mitochondrial volume) and fly health (resistance to various stresses, motility, longevity) will provide a direct test of whether boosting mitochondrial protein degradation can be sufficient to increase health and longevity.  These results will help us understand whether mitochondrial function is a limiting factor for aging and longevity.

Name of research group, project, or lab
Hur lab
Logistics Information:
Project categories
Biology
Student ranks applicable
First-year
Sophomore
Junior
Senior
Time commitment
Summer - Full Time
Compensation
Paid Research
Number of openings
2
Techniques learned

Fruit fly culture (feeding, collecting, mating, etc.)

quantitative PCR analyses using fly samples to measure gene transcription and DNA copy number

in vitro assays for mitochondrial function (protein degradation, different aspects of metabolism)

physiological assessments of fly health (resistance to stress, ability to climb, longevity)

 

Contact Information:
Mentor
Jae Hur
hur@hmc.edu
Principal Investigator
Name of project director or principal investigator
Jae Hur
Email address of project director or principal investigator
hur@g.hmc.edu
2 sp. | 0 appl.
Hours per week
Summer - Full Time
Project categories
Biology