Transdermal Transport

Project Background: Human skin provides a two-way barrier that prevents potentially harmful chemicals or diseases from entering the body while slowing water as it exits the body. The outermost layer of skin, called the stratum corneum (SC), presents the dominant resistance to transport; the SC is composed of many corneocytes (dead cells) in a lipid bilayer continuum in a brick-and-mortar structure. In order to reach the bloodstream, any molecule on the surface of the skin must pass through the SC, so understanding transport in the SC is key to transdermal drug delivery design and setting dermal exposure limits to toxic chemicals.  

 

Our Work:

  • In Vivo Transepidermal Water Loss (TEWL) Experiments: To examine the effects of humidity and/or stretching on transport through skin, we conduct in vivo (human volunteer) studies. We have designed a stretching apparatus for constant strain uniaxial extension, and are currently refining an instrument that can carry out constant strain and constant stress extension and obtain important mechanical properties (see below); for studying effects of extension, we measure TEWL using the BioX AquaFlux before and after extension. For examining the effects of humidity on transport, participants place their arms in a humidity-controlled chamber and we measure TEWL across humidity levels. 

 

  • Mechanical Stretching Device Design, Building, and Control: We are refining the design of a device that can measure the mechanical properties of skin for test subjects or dermatology patients. The device uses a combination of linear actuators and load cells and interfaces with a data acquisition instrument to digitally process output voltage signals from the load cells.  We hope to first validate the property measurement on subjects who do not have any disease affecting the mechanical properties of skin. We will then compare properties measured in a control (non-disease) group to those measured in a group of subjects who have a disease known to affect mechanical properties to determine if they show statistically significant differences.

 

  • Finite Element Modeling: We are using the finite element modeling (FEM) program COMSOL to run simulations of transport across the skin as the lipid and corneocyte dimensions change with hydration or mechanical extension. To determine the extent of changes in SC dimensions with mechanical stretching, we are developing a mechanical model of the skin structure, including layers deeper than the SC.

 

ESSAY PROMPT:

1.     A statement of your interest in research in general and in the project in particular.  Please include:

  • Why you are interested in this project,
  • Which parts of the project most interest you,
  • Information on your relevant background or desire to learn specified skills, and 
  • How many units you would be able to sign up for (typically between one and three) in Spring 2021 and Fall 2021 and briefly explain how adding these additional units per semester year would fit into your academic plan.  Note that each unit requires three hours of research work per week plus meetings.

2.   The names of two HMC professors (or outside research or internship advisors) who could provide references on your work style. Professors from project or lab classes might be especially good choices.

Name of research group, project, or lab
The Lape Lab: Transdermal Transport Team
Logistics Information:
Project categories
Biomedical Engineering
Biomedical Sciences
Chemical Engineering
Mechanical Engineering
Student ranks applicable
First-year
Sophomore
Junior
Student qualifications

This project involves both modeling and experimental work, and you are welcome to decide which combination of these aspects you would like to pursue (please note this in your statement).  If you would like to do experimental work, you should feel comfortable with tinkering with equipment; if you would like to focus on modeling, you should have a desire to understand the basics of mass transfer and to learn how to use COMSOL.

Please note that this position begins in Sp 2021 (for academic credit) and continues through Summer 2021 (for pay) and Fa 2021 (for academic credit).

Time commitment
Spring - Part Time
Summer - Full Time
Compensation
Academic Credit
Paid Research
Number of openings
2
Techniques learned

Finite element modeling (COMSOL), device design, mechanical property testing, testing with human subjects

Contact Information:
Mentor name
Nancy Lape
Mentor email
lape@hmc.edu
Mentor position
Faculty
Name of project director or principal investigator
Nancy Lape
Email address of project director or principal investigator
lape@hmc.edu
2 sp. | 0 appl.
Hours per week
Spring - Part Time (+1)
Spring - Part TimeSummer - Full Time
Project categories
Chemical Engineering (+3)
Biomedical EngineeringBiomedical SciencesChemical EngineeringMechanical Engineering